描述

The 7300Plus Real-Time PCR System delivers precise and quantitative results for a variety of genomic research applications, and is designed for both first-time and experienced users. Using the traditional halogen lamp and CCD camera, the 7300Plus Real-Time PCR System is a four-channel, 96-well (standard block) qPCR system. As an enhancement of the previous 7300 Real-Time PCR System, the 7300Plus system offers optimized optical performance with dye flexibility and a streamlined user experience.

>> Download Real-Time PCR Software v2.4

7300Plus Real-Time PCR System features include:
• Four-color platform calibrated for a broad range of dyes: FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/Cy™3, and ROX™/Texas Red®, and supporting a number of custom dyes: HEXTM, TETTM, ABY®, JUN®, etc.
• Upgraded optical system for enhanced excitation efficiency and multiplexing performance
• Advanced multi-component algorithm that minimizes spectral crosstalk—ideal for multiplex assays
• User-friendly software with Chinese-language option that includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward

Benefits of the 7300Plus Real-Time PCR System include:
• Affordable price for a high-performance instrument
• Compatibility with millions of TaqMan® assays for gene expression, genetic variation, and gene regulation experiments
• Easy-to-use software that helps to make operation and data analysis simpler

Real-Time PCR Software v2.4 with Chinese-language option
The 7300Plus Real-Time PCR System is equipped with the easy-to-use Real-time PCR Software v2.4. This software is an upgraded version of the 7500 Software v2.3 and includes many easy-to-use features, such as plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. This software also includes the Gene Expression Study package and a variety of melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates. Both English-language and Chinese-language versions of the software are available.

The Gene Expression Study package allows for greater flexibility and accuracy in your gene expression assays by enabling the following:
• Analysis of a large number of plates in one study
• Sorting of data by biological or technical replicate group
• Use of multiple endogenous controls
• Assay efficiency correction

Simple assay set-up
The intuitive software and protocol wizards help guide new users through their real-time PCR experiments.

Real-time data monitoring, dissemination, and storage
The 7300Plus Real-time PCR System measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle. Your data is stored on the computer connected to the instrument, and can be transferred via emails or USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.

Supports a variety of analysis methods:
• Standard curve (absolute quantitation)
• Relative standard curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt curve analysis

Supports many real-time PCR applications
The 7300Plus Real-Time PCR System supports a wide range of applications: gene expression analysis, microRNA analysis, SNP genotyping, pathogen quantitation, mutation detection, isothermal and positive & negative analysis.

To facilitate many of these applications, we provide pre-formulated, ready-to-use, quality-tested TaqMan® Assays for use with the 7300Plus system to help reduce your assay optimization efforts.

 

规格

Block Format:	Non-Interchangeable
Calibrated Dye:	Cy3™, FAM™, JOE™, NED™, ROX™, SYBR® Green I, TAMRA™, Texas Red®, VIC®
Dimensions:	Depth: 45 cm (17.72 in), Height: 49 cm (19.29 in), Width: 34 cm (13.39 in)
Dynamic Range:	9 logs
External Computer:	16 GB NON ECC 16000 MHz DDR3 Memory, 16X DVD, 2x500 GB HD, Dell Minitower: Intel Core i7-4770S (3.10GHz)
For Use With (Equipment):	7300Plus Real-Time PCR Instrument
Format:	96-well plate
High Throughput Compatibility:	Multiplexing
Input Voltage:	220V
Optics:	4 Color, CCD Camera, Tungsten-halogen lamp
Passive Reference Dye:	No ROX, ROX (Pre-mixed), ROX (Separate Tube)
Peak Block Ramp Rate:	2.5°C⁄sec
Power Limit:	950 W
Precision:	99.7% confidence (5000 & 10000 copies)
Product Size:	1 system
Reaction Speed:	Standard
Reaction Volume Range:	20-100 µl
Sample Ramp Rate:	1.1°C⁄sec
Sensitivity:	1 copy of RNase P gene (human genomic DNA)
Shipping Condition:	Approved for shipment at Room Temperature or on Wet Ice
Software Compatibility:	Primer Express, Real-Time PCR Software v2.4
Temperature Accuracy:	0.25°C (35°C to 95°C after 3 min)
Temperature Range (Metric):	4.0-99.9 °C
Temperature Uniformity:	0.50°C (after 30 sec)
Thermal Cycling System:	Peltier-Based System
Throughput:	4 to 5-96-well plates per 8-hr day
Weight:	75 lbs

内容及储存